Decreased motility of human spermatozoa presenting phosphatidylserine membrane translocation-cells selection with the swim-up technique

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Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa.

UNLABELLED The objectives of this cross-sectional observational study were: (i) to detect DNA damage and plasma membrane translocation of phosphatidylserine in purified sperm populations of high and low motility, and (ii) to analyse their relationship with the endogenous generation of reactive oxygen species. Ejaculates from infertile men were examined following gradient centrifugation. The mai...

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Characterization of the lectin binding pattern in human spermatozoa after swim-up selection.

Capacitation is characterized by a hyperactivated pattern of sperm motility. The acquisition of highly motility is present in the early stages of capacitation. Sperm progressive motility is one of the most important parameters for determining the suitability of semen for processing. However, previous studies have shown that some sperm showing good motility have membrane damage. The aim of our s...

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Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method

OBJECTIVE In 2014, enrolled 20 patients who applied to the Unit of Assisted Reproduction Techniques, Konya Necmettin Erbakan University. Based on the presence of hyaluronic acid (HA) in the oocyte-cumulus cell complex, sperm attached to HA in vivo were modeled in vitro. Available healthy sperm obtained in the swim-up procedure using HA were investigated. MATERIALS AND METHODS This observation...

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Motility evaluation of human spermatozoa

In this contribution we investigate the applicability of photon correlation techniques as a clinical tool for the evaluation of motility parameters of undiluted human sperm. Measurements on semen samples, selected visually for 'good' motility, yielded reproducible estimates of the fraction motile spermatozoa and the mean speed of the motile cells.

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Piperlongumine-Induced Phosphatidylserine Translocation in the Erythrocyte Membrane

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ژورنال

عنوان ژورنال: Human Cell

سال: 2011

ISSN: 1749-0774

DOI: 10.1007/s13577-011-0024-1